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KMID : 1159320150170030333
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Korean Journal of Vision Science
2015 Volume.17 No. 3 p.333 ~ p.341
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The effect of cycloplegic Cyclogylⓡ and Atropineⓡ on cytotoxicity in the conjunctiva cell
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Lee Young-Hwan
Seo Eun-Sun
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Abstract
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Purpose: Cyclogylⓡ and Atropineⓡ are in use as cycloplegic agents. This study aimed to investigate the effects of the cycloplegic agents on the conjunctival cell.
Methods: A conjunctival cell line was treated for 15 minutes with Cyclogylⓡ, cyclopentolate HCl, Atropineⓡ and atropine sulfate at their concentrations of 1%, 0.75%, 0.50%, and 0.25%, respectively, and the preservative of benzalkonium chloride at the concentrations of 0.01%, 0.0075%, 0.005%, and 0.0025%; and then the cell viability was measured, using the MTT assay. The morphological changes were investigated with an optical microscope after hematoxylin-eosin staining, and apoptosis was measured, using flow cytometry.
Result: It was found that the cell viability exceeded 70% only in 0.25% cyclopentolate HCl and 0.25% atropine sulfate. And as a result of the H-E staining, the cell count decreased and fragmentation of neuclei was observed in 1% Cyclogylⓡ and 1% Atropineⓡ. In 1% Cyclogylⓡ 1% and 1% Atropineⓡ, the A0 level was found to be 52.92% and 31.36%, compared with the contrast group, and apoptosis occurred.
Conclusion: It was found that in the cycloplegic agents of 1% Cyclogylⓡ and 1% Atropineⓡ, the cell viability does not exceed 30% and apoptosis occurs.
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KEYWORD
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Cycloplegic, Cyclogyl, Atropine, Conjunctival cell
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